Journal: Neuron
Article Title: Corticostriatal flow of action selection bias
doi: 10.1016/j.neuron.2019.09.028
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains AAV-DJ-EF1α-DIO-mRuby2-P2A-TVA Lim Lab N/A AAV-DJ-EF1α-DIO-RVG Lim Lab N/A EnvA-RVΔG-eGFP Lim Lab N/A RVΔG-GFP Lim Lab N/A RVΔG-tdTomato Lim Lab N/A CAV-Cre BioCampus N/A AAV1-CAG-tdTomato UPenn Vector Core Addgene: 105554-AAV1 AAV1-CAG-FLEX-tdTomato UPenn Vector Core Addgene: 51503-AAV1 AAV1-CAG-FLEX-eGFP UPenn Vector Core Addgene:51502-AAV1 AAV9-EF1α-DIO-eNpHR3.0-eYFP UPenn Vector Core Addgene: 26966-AAV9 Chemicals, Peptides, and Recombinant Proteins CTB-Alexa-Fluor 594 conjugate Thermo Fisher Cat# {"type":"entrez-nucleotide","attrs":{"text":"C22842","term_id":"2415898","term_text":"C22842"}} C22842 CTB-Alexa-Fluor 488 conjugate Thermo Fisher Cat# {"type":"entrez-nucleotide","attrs":{"text":"C22841","term_id":"2415897","term_text":"C22841"}} C22841 Experimental Models: Organisms/Strains Mouse: wild-type (C57BL/6) Charles River Laboratories Strain Code:027 Mouse: B6.Cg-Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze /J; also known as Rosa26-CAG-LSL-tdTomato The Jackson Laboratory Cat#007914 Mouse: B6.CBA-Tg(Camk2a-tTA)1Mmay/J; also known as Camk2a-tTA The Jackson Laboratory Cat#003010 Mouse: B6.DBA-Tg(tetO-GCaMP6s)2Niell/J; also known as tetO-GCaMP6s The Jackson Laboratory Cat#024742 Software and Algorithms MATLAB v. 2014a & 2019a MathWorks RRID: SCR_001622 ImageJ FIJI Schneider et al., 2012 RRID: SCR_002285 Bootstrap test Stout et al., 1999 N/A Spike-triggered mixture model Theis et al., 2016 https://github.com/lucastheis/c2s Lateral motion correction of calcium images Mitani and Komiyama, 2018 N/A Open in a separate window KEY RESOURCES TABLE PPC neurons projecting to STR (PPC-STR) and pM2 (PPC-pM2) form parallel subsystems.
Techniques: Plasmid Preparation, Recombinant, Software